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国家生物医学分析中心蛋白质组学技术平台

400-6699-117转1000

主要课题

• 国家863课题（2003AA233010）“蛋白质组学新技术在肿瘤泛素通路研究中的应用”。
• 973计划（2004CB520803）“人类肝脏蛋白质组定位图新技术新方法研究”
• 国家973计划基因治疗项目课题（2004CB518803） “定点整合、原位修复技术及机理的研究”
• 国家科技攻关计划项目（人类重大疾病及重要生理功能相关的蛋白质组学研究）“蛋白质定位技术平台建立与应用”课题。
• 北京市248项目（H030230280290）“肝再生中系列蛋白质复合体的研究”课题。
• 国家自然科学基金课题面上项目（30270299）“阻断泛素通路时Bcl-2蛋白质形成复合体的生物质谱分析”课题。
• 国家自然科学基金课题面上项目（20475062）“新型分子成像技术——质谱扫描成像及其应用”课题。
• 国家863计划课题（2001AA233041）“蛋白质组技术平台建立及其在肿瘤研究中应用”。
• 国家自然科学基金课题面上项目（30070377）“阻断泛素通路对正常和肿瘤细胞影响的巨大差异机制研究”。
• 国家863计划课题（2002AA232021）“应用蛋白质组技术对白血病细胞凋亡相关蛋白质的高通量鉴定”。
• 科技部创新课题“生物质谱技术对蛋白质复合体的鉴定”。
• 国家自然科学基金项目“异烟肼和利福平联合使用肝毒性增加的代谢组学、基因组学、蛋白质组学研究”。
• NSFC项目“胎儿酒精综合征发生机制的基础研究”。  

知识产权

取得2项发明专利：

1. eIF-5A及其与syntenin蛋白质复合物在医药领域中的应用。200310115020.5
2. 用于白血病分型的蛋白质分子标志物。200310115021.X

取得2项版权：

1. 基于WEB的蛋白质检索系统，登记号： 2003SR8991。
2. 基于WEB的精确肽质量指纹谱蛋白质鉴定系统, 登记号：2003SR8990。

近期论文摘录

1. Cui JW, Wang J, He K, Jin BF, Wang HX , Li W, Kang LH, Hu MR, Li HY, Yu M, Shen BF, Wang GJ, Zhang XM..Proteomics analysis of human acute leukemia cells: insight into their classifications. .Clinical Cancer Research, 2004，10(20):6887-96.
2. Li AL, Li HY, Jin BF, Ye QN, Zhou T, Yu XD, Pan X, Man JH, He K, Yu M, Hu MR, Wang J, Yang SC, Shen BF, Zhang XM. .A novel eIF5A complex functions as a regulator of p53 and p53-dependent apoptosis. .Journal of Biological Chemistry，2004，279(47):49251-8.
3. Cui JW, Wang J, He K, Jin BF, Wang HX , Li W, Kang LH, Hu MR, Li HY, Yu M, Shen BF, Wang GJ, Zhang XM..Two-dimensional electrophoresis protein profiling as an analytical tool for human acute leukemia classification. .Electrophoresis，2004，26(1):268-279.
4. Jin BF, He K, Wang HX, Wang J, Zhou T, Lan Y, Hu MR, Wei KH, Yang SC, Shen BF, Zhang XM. .Proteomic analysis of ubiquitin-proteasome effects: insight into the function of eukaryotic initiation factor 5A. .Oncogene，2003 22:4819-4830.
5. Xia Q, Wang HX, Wang J, Zhang JY, Liu BY, Li AL, Lv M, Hu MR, Yv M, Feng JN, Yang SC, Zhang XM, Shen BF. .Proteomic analysis of interleukin 6-induced differentiation in mouse myeloid leukemia cells..International Journal of Biochemistry and Cell Biology, 2005 (in press)
6. Jin BF, Wang HX, He K, Yu M, Hu MR, Yang SC, Shen BF, Zhang XM. .Proteomic analysis of apoptosis triggered by inhibition of ubiquitin-proteasome pathway in M07e leukaemic cells..Cell Research, 2002, 12:271.
7. Wang Hongxia, Zhang Xuemin，Yang SC, Xiao JH, Nie AH, Zhao LQ, Li S. .ESI-MS study on non-covalent bond complex of rhFKBP12 and new neurogrowth promoter.. Science in China, 2003, 46(3):286-292.
8. Lan Yu, Zhang Xuemin, Yang PD, Hu MR, Yu M, Yang Y, Shen Bf. .Effects of inhibition of ubiquitin-proteasome pathway on human primary leukemic cells. .Science in China，2002, 45(6):647-654.
9. Zhang XM, Hu MR, Lan Y, Yu M, Chen BD. .Cleavage of Bcl-2 Protein by Activated Caspase-3 Is Associated with Inactivation of Lyn(p53/56) Kinase Activity in Human M-07e Leukemic Cells during Apoptosis. .J. Experiment Hematolog. 2000, 8(3):166-175.
10. Zhang XM, Lin H, Chen C, Chen BD. .Inhibition of ubiquitin-proteasome pathway activates a caspase-3-like protease and induces Bcl-2 cleavage in human M-07e leukaemic cells..Biochem. J. 1999，340:127-133
11. Li AL, Li HY, Jin BF, Ye QN, Zhou T, Yu XD, Pan X, Man JH, He K, Yu M, Hu MR, Wang J, Yang SC, Shen BF, Zhang XM. .A novel eIF5A complex functions as a regulator of p53 and p53-dependent apoptosis..Molecular & Cellular Proteomics. 2004, 3(10): S76.
12. Cui JW, Wang J, He K, Jin BF, Wang HX , Li W, Kang LH, Hu MR, Li HY, Yu M, Shen BF, Wang GJ, Zhang XM. .Proteomics analysis of human acute leukemia cells: insight into their classifications..Molecular & Cellular Proteomics. 2004, 3(10): S23.
13. Yan X., Xue H., Liu, H., Hang, J., Wong J.T.F. and Zhu, G. . NMR studies of Bacillus subtilis tRNATrp hyperexpressed in Escherichia coli - Assignment of imino proton signals and determination of thermal stability..Journal of Biological Chemistry, 2000，275(10), 6712-6716
14. Yan, X., Kong, X., Xia, Y., Sze, K.H. and Zhu, G.J.. Determination of Internucleotide hJHN Couplings by the Modified 2D JNN Correlated-[15N, 1H]-TROSY. .J. Magn. Reson. 2000，147(2), 357-360
15. Wang, YQ., Yan, XZ., Gong, SS., Fu, WH. .Two New C - 21 Steroidal Glycosides from Cynanchum aurichulatum. .Chinese Chem. Lett. 2002，13(6): 543-546
16. Yao YN, Zhang QS, Yan XZ, Zhu G, Wang ED. .Substrate-induced conformational changes in Escherichia coli arginyl-tRNA synthetase observed by 19F NMR spectroscopy. . FEBS Lett. 2003,547(1-3):197-200
17. Yao YN, Zhang QS, Yan XZ, Zhu G, Wang ED. .Escherichia coli tRNAArg4 (UCU) induces a constrained conformation of the crucial X-loop of arginyl-tRNA synthetase..Biochem. Biophys. Res. Commun. 2004, 313: 129-134
18. Zhang, Q., Ye, W., Yan, X., Zhu, G., Che, C.T. and Zhao, S. .Cernuosides A and B, two sucrase inhibitors from Pulsatilla cernua. .J. Nat. Prod. 2000, 63(2), 276-278
19. Zhu, G., Xia, Y., Sze, K.H. and Yan, X. . 2D and 3D TROSY-enhanced NOESY of N-15 labeled proteins. . J. Biomol. NMR 1999, 14(4), 377-381
20. Sze, K.H., Yan X., Kong, X., Che, C.T. and Zhu, G. .Phase sensitive 3D J-resolved HMBC experiment for spectral assignment and measurement of long-range heteronuclear coupling constants. . Tetrahedron Lett. 1999, 40(30), 5587-5591
21. Zhu, G., Kong, X., Yan, X. and Sze, K.H. .Sensitivity enhancement in transverse relaxation optimized NMR spectroscopy. .Angew. Chem. Int. Edt. 1998, 37(20), 2859-2861
22. Xiaoxiao Chen, Yong Li, Kaihua Wei, Li Li, Wanli Liu, Yunping Zhu, Zongyin Qiu,and Fuchu He. .The Potentiation Role of Hepatopoietin on Activator Protein-1 Is Dependent on Its Sulfhydryl Oxidase Activity. .Journal of Biological Chemistry， 2003 ，278(49):49022
23. Yong Li, Kaihua Wei，et al. .Identification of hepatopoietin dimerization, its interacting regionsand alternative splicing of its transcription. Eur.J.Biochem. 2002 ，269：1. Eur.J.Biochem. 2002 ，269：1